What is Protein Tomography?

Protein Tomography is an innovative approach to three-dimensional (3D) protein visualization, to produce images of individual proteins and/or macromolecular complexes—including integral membrane proteins.

With Okinawa Protein Tomography, it is now possible

I. To approach the study of individual proteins as well as protein complexes, revealing properties such as shape, binding domains and flexibility,

II. To reveal conformational distribution of flexible molecules, by taking images of individual proteins and protein complexes,

III. Visualization of membrane proteins. It is now the only method that can provide this information,

IV. To analyze proteins in a variety of contexts

Some features of our technology:

1. Structural analysis of individual molecules
➡ not the averaged molecular structure, possibility to obtain dynamic structure

2. Reduction of electron beam irradiation and direct electron detecto
r➡ analysis without destroying the molecular structure

3. Analysis by COMET (constrained maximum entropy tomography)
➡ improvement of image quality by noise removal

More about Protein Tomography here